- Light Independent Reaction -
The Calvin Cycle -
The products of the light dependent reaction is ATP and reduced NADP // both used to reduce glycerate-3-phosphate.
This would have occur with or without light.
1. The CO₂ diffuses into the leaf through the stomata // dissolves in water around the walls of mesophyll cells // diffuses through the cytoplasm and chloroplast membrane into the stomata of chloroplast.
2. In stroma CO₂ reacts with 5 carbon compound // catalysed by enzyme ribulous bisphosphate carboxylase (Rubisco)
3. Reaction CO₂ + RuBP = 2X 3-carbon glycerate 3-phosphate.
4. Reduced NADP from light dependent reaction, reduces glycerate 3-phosphate ⇒ triose phosphate using energy supplied by ATP.
5. NADP is re-formed // goes back into the light dependent reaction = gaining protons.
6. Triose phosphate converted into organic substances // starch, cellulose, lipids, glucose, amino acids, and nucleotides.
7. Most triose phophate is used to generate ribulose bisphosphate // ATP is used from Light Dependent Reaction .
Site of the light-independent reaction -
Chloroplast is adapted in carrying out the light independent reaction of photosynthesis.
The stroma contains a fluid with all the enzymes needed for the light independent reaction. Stromal fluid is membrane-bound in the chloroplast.
The stroma fluid surrounds the grana // products of light dependent stage in grana can diffuse into stroma
Contains DNA and ribosomes // can quickly and easily manufacture some of the proteins involved in the light-independent reaction.
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